Journal article · Electrochimica Acta 2019
Towards an Optimal Dose-Response Relationship in Gene Electrotransfer Protocols
Electrochimica Acta, 2019
Abstract
In search of an optimal gene electrotransfer (GET) protocol, an electroporation-based (EP) tumor treatment with great potential as a non-viral gene-delivery system, the concept of the dose-response relationship is introduced. It is shown that a reliable dose parameter is the pulse dosage and reliable response parameters are the reversibly electroporated tissue area as well as the unwanted damaged tissue area and plasmid damage due to pH.
An optimal dose-response relationship in a GET protocol is predicted as the critical pulse dosage yielding maximum reversibly electroporated tissue area with minimal tissue area damage induced by pH fronts. Moreover, since damage induced by pH changes is proportional to the Coulomb dosage, damage induced by pH fronts is negligible in typical EP-based tumor protocols such as in electrochemotherapy (ECT) and irreversible electroporation (IRE) but not in GET, due to the most often longer pulses applied.
The version of record is at doi:10.1016/j.electacta.2019.07.029.
Cite this paper
Download .bib ↓Emmanuel Lujan, Matías Marino, Nahuel Olaiz and Guillermo Marshall, “Towards an Optimal Dose-Response Relationship in Gene Electrotransfer Protocols,” Electrochimica Acta, 2019.
doi:10.1016/j.electacta.2019.07.029
@article{lujan2019towards,
title={Towards an Optimal Dose-Response Relationship in Gene Electrotransfer Protocols},
author={Luj{\'{a}}n, Emmanuel and Marino, Mat{\'{i}}as and Olaiz, Nahuel and Marshall, Guillermo},
journal={Electrochimica Acta},
year={2019},
doi={10.1016/j.electacta.2019.07.029}
}